Analysis of PAHs in soil according to EPA 8310 method with UV and fluorescence detection


Полициклические ароматические углеводороды (ПАУ) (нафталин, аценафтилен, аценафтен, флюорен, фенантрен, антрацен, флюорантен, пирен, 1,2-бензо(а)антрацен, хризен, бензо(б)флюорантен, бензо(к)флюорантен, бензо(а)пирен, дибензо(а,х)антрацен, бензо(г,х,и)перилен, индено[1,2,3-цд]пирен)

Объекты Почва

Англоязычная прикладная статья — Analysis of PAHs in soil according to EPA 8310 method with UV and fluorescence detection

Оборудование Система Agilent 1260 с флуориметрическим детектором и УФ-детектором (для анализа аценафтилена)

Burning carboniferous materials releases copious amounts of smoke containing polynuclear aromatic hydrocarbons (PAHs). These PAHs were declared by the US Environmental Protection Agency (US EPA) as priority pollutants, suspected to be carcinogenic. This Application Note describes a reproducible method according to EPA 8310 for sample preparation and HPLC analysis of PAHs in soil with good recovery rates, and detection limits that fulfill regulatory requirements.

The HPLC method included both diode array and fluorescence detection modes. The method was developed on an Agilent 1260 Infinity LC system using a 4.6 × 150 mm, 5 μm column and validated including robustness testing. This conventional method was then transferred to an Agilent 1290 Infinity LC System using a 2.1 × 50 mm 1.8 μm column. Both methods were compared regarding precision, limit of detection (LOD) and limit of quantitation (LOQ).

Скачать pdf (927.47 кб)
Используемое оборудование